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United States Department of Agriculture

Agricultural Research Service

Research Project: Improvement of Biotic Stress Resistance and Quality Traits in Soybeans

Location: Corn, Soybean and Wheat Quality Research Unit

Project Number: 5082-21000-016-00
Project Type: Appropriated

Start Date: Nov 15, 2013
End Date: Nov 14, 2018

Objective:
Objective 1: Develop screening methods, identify soybean genotypes with insect resistance, characterize resistance mechanisms, and develop germplasm adapted to the Northeastern U.S. with resistance to insects. Sub-objective 1A. Develop screening methods for evaluation of genetic resistance of soybeans to brown marmorated stink bug (BMSB), use selected methods to screen soybean germplasm/cultivars to identify lines with resistance to BMSB, and characterize resistance mechanisms (critical needs; non-hypothesis driven). Sub-objective 1B. Pyramid antibiotic and antixenotic soybean aphid resistance genes in U.S. adapted soybean lines. Objective 2: Map novel quantitative trait loci (QTL) for seed protein contents and develop high-yielding soybean germplasm with improved seed quality. Sub-objective 2A. Map QTL for seed protein and oil content in PI 205085 and PI 253666A and develop molecular markers closely linked to novel QTL. Sub-objective 2B. Combine high seed protein content and high-yield in MG III U.S. adapted soybean germplasm.

Approach:
Develop genetic resources for increasing the profitability and sustainability of the U.S. soybean crop and to make it more competitive in international markets. Stress resistant crops are extremely useful for environmentally sustainable, economically viable production and integrated pest management systems. Developing cultivars with genetic resistance to biotic stresses involves: (i) identification of new sources of resistance by screening germplasm, (ii) characterization of the resistance genes and (iii) integration of the resistance genes into desired cultivars or elite breeding lines. The USDA Soybean Germplasm Collection at Urbana, IL has nearly 20,000 soybean genotypes, most of which are poorly adapted plant introductions (PIs) from other countries. These PIs are often important sources of resistance to biotic stress. However, integration of resistance genes from PIs into adapted cultivars is often associated with yield reduction and other deleterious effects (linkage drag) due to transfer of undesirable alleles from the PIs that are tightly linked to the resistance genes. Marker assisted breeding (MAB) can be used to reduce the transfer of such undesirable alleles. In addition, MAB can reduce the time needed to transfer genes from PIs to cultivars.

Last Modified: 12/18/2014
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