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United States Department of Agriculture

Agricultural Research Service

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Research Project: Biology and Spread of Grapevine Red Blotch-Associated Virus

Location: Crops Pathology and Genetics Research

Project Number: 2032-22000-015-30
Project Type: Reimbursable

Start Date: Apr 15, 2013
End Date: Apr 14, 2015

Objective:
1)Investigate the role of Grapevine red blotch-associated virus in red blotch disease. 2)Monitor the spread of Grapevine red blotch-associated virus in selected vineyards. 3)Determine the population structure and genetic variability of Grapevine red blotch-associated virus to optimized diagnostics. 4)Disseminate research results to farm advisors and the industry.

Approach:
1)Using Cabernet Sauvignon vines that were graft inoculated with GRBaV-infected material in 2011, determine if symptoms develop and how long it takes for symptoms to manifest. Plants will be visually observed at two-week intervals for symptoms developments and at monthly-intervals for virus detection. Data will be collected and interpreted to determine if GRBaV is the etiological agent of red blotch disease. 2)Monitor the incidence of GRBaV in a newly established vineyard adjacent to a GRBaV-infected vineyard to verify if horizontal spread of the virus is able to occur. 3)Test a set of 50 additional GRBaV isolates from varied cultivars and geographic origin to gain a better insight into the genetic variability of GRBaV. New primers will be validated with a collection of GRBaV-infected vines from different cultivars and geographic origin. 4)Progress will be communicated to industry via various avenues.

Last Modified: 10/24/2014
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