The Differential Gene Expression of the Gene's Organ in the Cattle Fever Tick, Rhipicephalus Microplus
Tick and Biting Fly Research
Project Number: 3094-32000-036-43
Specific Cooperative Agreement
Start Date: Sep 09, 2013
End Date: Nov 30, 2014
The objective overall of the ARS project is to mine the genome of R. microplus to develop novel tick control technology. The Gene's organ is responsible for adding a wax coating to the eggs during ovipositing that protects the eggs from desiccation, fungal and bacterial infections, and harmful chemical. Previous research has shown that disruption of this organ would be a viable novel tick control method. By sequencing the transcriptome of the Gene's organ, 1) we will be adding to the Cattle Tick Genome Database; 2) identifying targets for organ development disruption; and 3) better understanding of the biology of the Cattle Fever Tick.
Dissect Gene's organs at separate developmental stages from Rhipicephalus microplus at the ARS-Mission laboratory. Extract RNA to synthesize cDNA. Send the cDNA of the samples to UTPA for sequencing. Data will then be analyzed by scientists at UTPA, the ARS-Kerrville laboratory, and the ARS-Mission laboratory.