Location: Foreign Animal Disease Research
Project Number: 8064-32000-056-17
Start Date: Aug 01, 2013
End Date: Jul 31, 2018
Specific host proteins which interact with viral proteins will be identified by USDA, ARS using a yeast two hybrid system. The proteins which demonstrate positive interaction will be further analyzed by INIA in ASFV infected cells using different methodologies including; coimmunoprecipitation, mammalian two hybrid system and confocal immune fluorescence. These methodologies will provide further characterization of the virus-host protein interactions. The cellular metabolic pathways involved in these interactions will be analyzed in order to gain an understanding of the possible role of the host-virus interaction.