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United States Department of Agriculture

Agricultural Research Service

Research Project: Exploring Novel Approaches to Reduce the Impact of Fusarium Head Blight and Don Project Number: 0500-00053-003-60
Project Type: Grant

Start Date: Jul 01, 2013
End Date: Jun 30, 2015

Objective:
(1) Identify and characterize other non-TRI cluster genes that are involved in protecting F. graminearum against trichothecene mycotoxins. (2) Test the feasibility of applying host-induced gene silencing (HIGS) to control FHB.

Approach:
(1) a) identify and characterize genes that are specifically induced by DON (named DIG genes; 2) generate and identify DOS mutants that have increased sensitivity to DON; and 3) express the DIG and DOS genes in the budding yeast and determine their functions in conferring DON tolerance to yeast cells. (2) a) determine whether synthetic siRNA oligos can be effectively taken up by F. graminearum and result in the silencing of specific fungal genes; 2) use the VIGS (virus-induced gene silencing) approach to express silencing constructs of F. graminearum genes in wheat and examine their effects on fungal infection and DON production; and 3) determine whether treatments with combinations of siRNA oligos or co-expression of different BSMV VIGS constructs targeting at multiple genes have synergistic effects on reducing fungal growth, virulence, or DON production.

Last Modified: 9/20/2014
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