PH/GBA3-Dependent Stability of D3G's Glycosidic Bond and the Potential Risk to Human Health
Project Number: 0500-00053-003-59
Start Date: Jul 01, 2013
End Date: Jun 30, 2014
(1) Develop appropriate approaches (e.g. HPLC, LC/MS/MS, and other pharmacology/molecular biology techniques) to determine the levels of DON and D3G in agricultural products and to determine the rate of D3G deconjugation and relative concentration of D3G vs. DON during human digestion and metabolism, (2) Determine the toxic effects and mechanisms of D3G and DON (and other related metabolites) in humans.
(1) Survey D3G and DON level from samples of barley, durum wheat and hard red spring (HRS) wheat, (2) Validate pH-dependent and GBA-catalyzed hydrolysis of D3G, and (3) Characterize metabolic products of D3G in human parietal and hepatocyte cells.