Location: Foreign Animal Disease Research
Project Number: 8064-32000-056-16
Start Date: Jun 01, 2013
End Date: May 31, 2016
1. Infected tissues will be characterized to identify the cell types responsible for virus replication in the pre-viremic phase of infection. These cells will be stained using several cell markers. Sections will be processed with different primary and secondary antibodies and examined using confocal microscopy. 2. Comparison of transcriptional and proteomic profiles between the attenuated and virulent parental viruses may provide critical information about innate mechanisms mediating early protection after vaccination. Detection will be performed using tonsils, mandibular lymph nodes, retropharyngeal lymph node and spleen. Dection will be conducted by confocal immunofluorescence microscopy and examination of the differential gene activation patterns by real time RT-PCR. 3. The effect of different PCM when used as therapeutics in ASFV infected animals will be assessed. Host factors differentially expressed during infection with attenuated ASFV strains will be assessed as therapeutics in ASFV infections. The host factors will be cloned in effect of the different PCMs on the replication of ASFV will be first tested in vitro; using primary cell cultures of swine macrophages. The effect of PCMs will be assessed in terms of reducing the virus yields. Those PCMs showing a significant effect in vitro will be further tested in vivo.