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United States Department of Agriculture

Agricultural Research Service

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Research Project: Protection of Chickens from Avian Influenza (Ai) Following Vaccination with Recombinant Bacillus Expressing Epitopes from Ai Virus Proteins

Location: Exotic and Emerging Avian Viral Diseases Research Unit

Project Number: 6612-32000-062-04
Project Type: Trust

Start Date: Apr 01, 2013
End Date: Mar 29, 2015

Objective:
1. To evaluate protection from low pathogenic avian influenza (AI) using a novel recombinant Bacillus vaccine expressing epitopes from the AI HA, NP and M2e proteins. 2. To characterize the serologic response following challenge.

Approach:
A Bacillus-vectored vaccine expressing epitopes from the AI HA, NP and M2e proteins will be generated by PGT (Pacific GeneTech Limited, Hong Kong). Groups of 8-10, one-day-old commercial chickens will be injected with inactivated bacillus-AI vaccine. After 21 days, the birds will be transported to SEPRL for challenge with LPAI H7N2 under BSL3Ag conditions. Protective immunity will be evaluated by morbidity /mortality to challenge, with oral and cloacal swabs taken to determine viral shedding. Humoral immunity will be evaluated by ELISA and/or HI.

Last Modified: 9/23/2014
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