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United States Department of Agriculture

Agricultural Research Service

Related Topics

Research Project: Zebra Chip and Potato Exports: Pest Delimitation and Vector/pathogen Biology Impacting Trade to the Pacific Rim

Location: Fruit and Vegetable Insect Research

Project Number: 5352-22000-020-19
Project Type: Reimbursable

Start Date: Jul 01, 2013
End Date: Jun 30, 2015

Objective:
Determine whether psyllids other than the potato psyllid, aphids, and leafhoppers are capable of transmitting zebra chip disease of potato. In addition, investigate the importance of zebra chip transmission through infected tubers.

Approach:
Lab colonies of psyllids other than potato psyllid, aphids, and leafhoppers will be established from field-collected insects. The insects will be assayed for the ability to transmit Candidatus Liberibacter solanacearum (Lso) following exposure to infected plants. The insects will then be dissected and their tissues will be hybridized with fluorescent probes which bind specifically to Lso. Tissue-specific infection by Lso will be observed using fluorescence microscopy. In addition, seed lots of several potato varieties of export importance will be infected with different haplotypes of Lso, monitored for emergence and Lso infection in above ground and tubers. Furthermore, it will be determined whether potato psyllids can acquire Lso from these plants.

Last Modified: 9/21/2014
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