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United States Department of Agriculture

Agricultural Research Service

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Research Project: Improving Catfish Broodstock Management by Manipulating Diet, Stocking Densities and Sex Ratios

Location: Warmwater Aquaculture Research Unit

Project Number: 6402-31000-009-18
Project Type: Reimbursable

Start Date: Jan 01, 2013
End Date: Mar 01, 2014

Objective:
Determine effects of male:female ratio, stocking density, and post-spawning consolidation on spawning success in channel catfish.

Approach:
This research will take place in 3 phases over 36 months, with each 12 month period being devoted to each of the 3 spawning trials. The first trial will determine the effects of male to female ratios of 1:1 and 1:4 on spawning success in channel catfish. Broodfish from a strain being developed as part of the USDA-ARS’s breeding program will be used in the study. Broodfish will be randomly assigned to treatments and stocked into 0.1 ha spawning ponds, with 6 replicate ponds per treatment. Ponds in both treatments will be stocked at approximately 1100 kg/ha and the number of males and females in each pond will be adjusted to the proper sex ratio. The second trial will compare stocking rates of 1100 and 2200 kg/ha on reproductive success of channel catfish. At stocking brood fish will be randomly assigned to each treatment and stocked into 0.1 ha spawning ponds, with 6 replicate ponds per treatment at a sex ratio of 1:1 for both treatments. The third trial will determine effects of consolidating broodfish at end of the spawning season on spawning the following year. Fish from a large, unrelated spawning trial stocked at approximately 1100 kg/ha will be used in this study. Fish from that trial will be harvested at the end of spawning (end of June) and a portion will be restocked at 2200 kg/ha (consolidated) and an additional portion will be restocked at 1100 kg/ha (control). At stocking brood fish from each treatment will be stocked into 0.1 ha spawning ponds, with 6 replicate ponds per treatment at a sex ratio of 1:1 will be used for both treatments. In all trials, spawning cans will be placed in each pond on April 1 and cans will be checked for spawns through mid-July. Spawns will be taken to the hatchery, weighed, sampled to determine number of eggs, and placed in a hatching tank. At hatch, fry will be enumerated volumetrically and the percent hatch will be determined for each spawn. Treatments will be compared for number of spawns, weight of spawns, percent hatch, average date of spawn, and survival of broodfish. DNA markers will be used to determine parentage of spawns. Economic analysis will determine relative cost/benefits for treatments in each trial.

Last Modified: 7/25/2014
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