Project Number: 6645-21220-014-00
Project Type: Appropriated

Start Date: Jan 09, 2013
End Date: Dec 31, 2013

Objective:
1. Identify genes and genome regions controlling key traits in diverse maize germplasm using multiple populations and novel genetic mapping methods. 2. Identify and characterize new sources of resistance to southern leaf blight (SLB), gray leaf spot (GLS), northern leaf blight (NLB), and Fusarium ear rot by developing new near-isogenic line sets, identifying QTL conferring resistance to multiple foliar diseases, and evaluating the known genetic diversity among public maize inbreds. 3. Incorporate favorable alleles from exotic maize into adapted maize lines, with particular emphasis on improvement of resistance to Fusarium ear rot and fumonisin contamination.

Approach:
Develop genetic mapping populations appropriate for identification of favorable alleles in exotic maize by identifying genome regions and specific genes controlling the response to photoperiod in multiple tropical maize inbreds. Develop interconnected multiple populations and novel statistical methods to map specific genes controlling quantitative trait variation in diverse maize. Fine-map QTL for SLB, NLB, GLS, Fusarium ear rot and fumonisin contamination resistance. Characterize specific disease resistance QTL using near-isogenic line pairs. Identify QTL conferring resistance to multiple foliar diseases. Complete multiple environment screening of a 302-line population encompassing the known genetic diversity among public maize inbreds for resistance to SLB, NLB and GLS. Identify new sources of resistance to SLB, Fusarium ear rot, and fumonisin contamination from the GEM program and the NCSU tropical maize breeding program. Develop new lines with elite agronomic performance and enhanced resistance to Fusarium ear rot by backcrossing. Test if selection for resistance to Fusarium ear rot results in improved resistance to contamination by the associated mycotoxin fumonisin.