Location: Vegetable Crops Research Unit
Project Number: 3655-21000-062-24
Start Date: Oct 01, 2012
End Date: May 30, 2015
1) Development of SNP- and SSR-based high-resolution genetic map: a. Develop a mapping population with 175 RILs from Gy14 × 9930 mating b. SNP genotyping of this population by re-sequencing c. Develop a SNP- and SSR-based high-resolution linkage map with this population d. Anchoring cucumber draft genome scaffolds onto this genetic map to develop an improved chromosome-level genome assembly; 2) QTL mapping of downy mildew and powdery mildew resistances: a. Resistance sources: PI 197088, PI 330628, Gy14 (dm-1) b. Mapping populations: F2 and F2:3 from different crosses c. Phenotyping: greenhouse artificial inoculation, and field trials (both ARS and sponsor) d. Genotyping: SSRs, indels, SNP-based CAP, dCAPS e. Fine mapping of major QTL for downy mildew and powdery mildew resistances, and clarify the linkage relationships of major QTLs for the two resistance genes.