Location: Vegetable Research
Project Number: 6659-22000-025-05
Start Date: Sep 01, 2012
End Date: Aug 31, 2016
Survey, sample collection in 2-3 countries in different continents through collaboration with seed pathologists with at least 3 samples per location. (3 x 6 x 3 x 3 = 162 samples);sRNA library preparation for a total of 162 preparations; Illumina sequencing (Cornell genomic center): libraries can be multiplexed for saving costs; Computer-based sequence analysis of small RNAs for virus identification; Confirmation of viral sequence by Sanger-sequencing; Characterization and molecular detection of new viruses and viroids; Characterization of novel viruses or viroids; Determine the seed transmissibility of new viruses; Joint development of detection technologies(antibody or primers for polymerase chain reaction). Using microRNA or a virus-induced gene silencing system to conduct functional gene analysis on the identified putative disease resistance candidate genes; Evaluate SNP mutations between resistant and susceptible parent lines and their association with disease resistance in the segregating populations (F1, F2, BC1); Molecular marker (CAPS) development; Selection and generation of breeding materials to achieve a multiple virus resistance through gene pyramiding using marker assisted selection; Evaluate potential economical losses due to virus disease infection; Conduct feasibility study on the need in developing a new technology and new cultivar; Determine economical benefits in adaptation of the new technology. Create a project website for the understanding of a global distribution of viruses and viroids in tomato; Educate the public on the new technology for disease diagnosis, virus detection and disease resistance; Develop a workshop to teach tomato breeders in using marker assisted selection; Develop an extension booklet such as focus on tomato.