Project Number: 6607-21000-011-03
Project Type: Specific Cooperative Agreement

Start Date: Aug 01, 2012
End Date: Sep 30, 2013

Objective:
1. The production of capsaicinoid profiles (UPLC-MS) of ~350 genebank accessions of Capsicum baccatum. 2. The elimination of PMMoV from 89 infested and 17 untested accessions of Capsicum frutescens from the USDA genebank. 3. The production of clean selfed seed from these and an additional 32 uninfested C. frutescens accessions.

Approach:
Greenhouse selfed (virus-free) seed, descriptor data and capsaicinoid profiles will be made available to the NPGS Vegetable Crops Curator. Three hundred and fifty fruit samples of Capsicum baccatum prepared previously will be extracted and analyzed via UPLC-MS to produce capsaicinoid profiles. Seed (inbred) of these accessions will be shared with the USDA genebank. Virus will be eradicated from infected C. frutescens seed lots as follows: Seed of PMMoV-infected accessions will be treated with 10% trisodium phosphate for 2 hours and rinsed. Seedlings will be screened for the presence of PMMoV coat protein by ELISA and rescreened after transplanting. Thirty-two uninfected accessions will also be included. For all accessions, plants representing each morphological type observed will be transplanted to the greenhouse and selfed seed produced from them.