INNOVATIVE PATHOGEN DETECTION AND CHARACTERIZATION TECHNOLOGIES FOR USE IN FOOD SAFETY
Molecular Characterization of Foodborne Pathogens
Project Number: 1935-42000-072-02
General Cooperative Agreement
Start Date: Jun 01, 2012
End Date: May 31, 2014
1: Develop and integrate operational technologies to rapidly and effectively concentrate viable target cells from food matrices in a self-validating system into an automated instrument.
1A: Integrate technology platforms that we have developed and individually tested into a usable technology for detecting L. monocytogenes in less than 8 h (time-to-result).
1B: Integrate technology platforms, currently being developed in our laboratory, into a usable technology for detecting Salmonella .
1C: Integrate technology platforms into a usable technology for detecting STEC.
2: Develop, evaluate, and adopt novel technologies for rapid detection, identification, and quantification of viable and non-viable target microorganisms. Research areas to be addressed include microfluidic biochips, optical light scattering technology, bacteriophage sensors, and Raman spectroscopy.
2A: Microfabricate and characterize microfluidic biochips that will direct, concentrate, and quantify living microorganisms using micro- and nano-scale electrical, mechanical, and optical methodologies.
2B: Develop light scattering technologies for rapid and high throughput detection and identification of pathogenic bacteria based on unique scattering signatures generated by concentrated colonies.
2C: Develop bacteriophages carrying reporter genes for the detection of E. coli O157:H7 and other foodborne pathogenic bacteria.
2D: Develop a highly sensitive enhanced Raman spectrosensor for field-deployable and routine benchtop in-lab identification of foodborne pathogens.
The approach focuses on four main components including separation, detection, identification, and quantification of target microorganisms from food matrices.