Project Number: 5348-21000-029-00
Start Date: Apr 22, 2013
End Date: Apr 21, 2018
New varieties and germplasm will be developed from pure lines selected from among segregating populations of peas, lentils, and chickpeas. Cyclical hybridization will be conducted to combine favorable alleles for traits of interest. Parental lines will include adapted germplasm, commercial cultivars and accessions from the various international breeding programs. Promising breeding lines will be released as either germplasm or varieties based on a rigorous comparison of their performance relative to that of commercial check varieties. Linkage analysis and the detection of associations between markers and different traits will be done using simple sequence repeats (SSRs), expressed sequence tagged- SSRs, and single nucleotide polymorphisms (SNPs). Pea recombinant inbred line (RIL) populations will be developed from a cross between Aragorn and Kiflica to identify markers associated with seed mineral concentrations. RILs from a cross between the pea cultivars Medora and Melrose will be used to identify markers associated with cold tolerance. Molecular markers associated with seed size and early maturity in chickpea will be detected using a RIL population developed from an interspecific cross between C. arientinum line Flip 90-27 and PI599072 (C. reticulatum). Associations between markers and quantitative trait loci (QTL) conditioning traits of interest will be detected by composite interval mapping. Improved methods will be developed to screen chickpea for reaction to Ascochyta blight. Toxins will be purified from liquid cultures of A. rabiei. Toxins will be adjusted to various concentrations and applied to detached chickpea leaflets. Leaflets treated with water will be used as controls. The speed of lesion development and final lesion size will be used to compare the reactions of different chickpea genotypes. The relationship between field disease scores of the chickpea genotypes and their sensitivity to the toxin will be determined. Studies to develop more efficient methods to screen peas and lentils for reaction to Sclerotinia white mold will initially examine resistant and susceptible materials reported in prior studies. Plants will be grown in the greenhouse and inoculated with agar plugs containing mycelia of S. sclerotiorum. Disease reaction will be scored by measuring the length of the lesion produced by the fungus over different time points. Two approaches will be taken to investigate the genetic factors responsible for pathogenicity and virulence of S. sclerotiorum. One approach will be to use Agrobacterium mediated transformation (AMT) to generate random mutations that will be screened to detect mutants with reduced virulence. The other approach will be to identify genes of S. sclerotiorum that are differentially expressed during the processes of infection and disease development.