Interfacing Classical Breeding and Genomics Towards Diversification of the Genetic Base of Potato Germplasm for Late Blight Resistance
Genetic Improvement of Fruits and Vegetables
Project Number: 8042-21000-273-08
Specific Cooperative Agreement
Start Date: Jun 26, 2012
End Date: Sep 30, 2015
To identify and characterize late blight resistance mechanisms from two diverse parents by RNA-Seq technology and combine multiple resistance mechanisms into a single elite donor line. Reduce the impact of late blight through the use of resistant varieties and reduced fungicide programs; Conduct late blight resistance phenotyping of advanced breeding lines and a breeding population for future use in genetic marker development.
Intercross late blight resistant clones of Solanum phureja-S. stenotomum with late blight resistance backcross introgression line of S. tuberosum-S. bulbocastanum. Construct RNA-Seq library for resistant and susceptible parents. Sequence transcriptome libraries.