Project Number: 6645-21220-012-59
Project Type: General Cooperative Agreement

Start Date: Apr 01, 2012
End Date: Mar 31, 2014

Objective:
Perform genetics analysis of high oleic acid lines.

Approach:
We plan to cross PI 603452 (GmFAD2-1a mutant) with each of PI 210179, PI 283327, PI 567189A, PI 567189B, and PI 578451 (GmFAD2-1b mutants) identified by our group to create a double mutant line containing even higher levels of 18:1. Simultaneously, single nucleotide polymorphism markers will be designed that correspond to each GmFAD2-1 mutation to increase the precision and speed by which the resultant high oleic phenotype can be introgressed into elite germplasm. For each cross, segregating populations will be developed and field trials studies will be conducted to quantify the extent by which the combinations of these new mutations enhance 18:1 content, and also establish the stability of the trait across different environments. The two mutant alleles will also be backcrossed to a high yielding line.