Project Number: 5306-21000-017-11
Project Type: Trust

Start Date: Jan 01, 2012
End Date: Dec 31, 2012

Objective:
1)SNP marker discovery and genotyping using next-generation sequencing platforms, 2)development and trait evaluation of genetic mapping and mutant populations, 3)Transcriptome sequencing in rice to identify genes and markers of interest for stress tolerance, disease resistance, grain quality, and yield.

Approach:
Single nucleotide polymorphisms (SNPs) will be identified by sequencing reduce representation libraries of selected rice germplasm in conjunction with computational genomics. SNP markers identified in this manner will be used to assess genetic diversity and map traits of interest where possible. Transcriptomes of various tissues and developmental stages of selected rice germplasm will be characterized by sequencing to identify genes of interest and potentially useful markers. In addition, rice plants undergoing cold stress during the reproductive stage of growth will be characterized using this method which will facilitate identification of rice genes involved in responses to this stress. Development of genetic mapping and mutant populations will consist of generation advance through single-seed descent. Screening populations for agronomic traits of interest will be performed in consultation and conjunction with Rice Experiment Station breeders.