Location: Sustainable Perennial Crops
Project Number: 1245-21220-252-02
Start Date: Aug 15, 2012
End Date: Aug 14, 2015
Pyrosequencing sequences small DNA fragments but is capable of sequencing very large numbers of small sequences in a very short time. Bioinformatic techniques allow for the assembling of these short sequences into larger sequences and ultimately into linkage groups. This can be done at progressively lower cost as techniques are improved. The transcriptome Oncobasidium theobromae will be determined using this technique. Since Oncobasidium theobromae cannot be cultured, our approach will be to isolate total RNA from infected cacao stems and sequence using pyrosequencing and other techniques. Colletotrichum gloeosporioides can be cultured. For this objective we will obtain both pure cultures and infected stem tissues. Total RNA will be isolated from these samples and sequenced. Finally, for the third objective, linkage mapping based on SNP markers will be applied to identify QTLs responsible for resistance/tolerance. Two mapping populations from different parental clones will be used.