The Orchestrated Role of Three Multifunctional Luteovirus Proteins in Virus Movement Within Plant Hosts and Aphid Vectors
Biological Integrated Pest Management Unit
Project Number: 8062-22000-021-20
General Cooperative Agreement
Start Date: Mar 15, 2012
End Date: Mar 14, 2015
The two luteovirid structural proteins, capsid protein (CP) and the readthrough protein (RTP), and the nonstructural P17 movement protein each contain multiple functional domains that interact either in cis or in trans with various plant, aphid, and/or aphid symbiont proteins to direct the overall virus transmission process. Our objective is to identify how these three proteins orchestrate the movement of virus within the plant and the aphid to facilitate the transmission of virus between hosts. Specific to this subcontract of the larger NIFA grant are experiments on the P17 protein and on the discovery of siRNAs in aphids that are viral in origin.
P17 mutants of Potato leafroll virus are generated and used in co-immunoprecipitation experiments to identify plant and aphid proteins that can interact with P17. These protein samples are forwarded to Ithaca for MS analysis. siRNA populations are characterized in extracts from whole aphids and specific aphid tissues after they have fed on purified virus, infected plants or healthy plants.