Project Number: 1245-22000-274-00
Project Type: Appropriated

Start Date: Mar 16, 2012
End Date: Mar 15, 2017

Objective:
Objective 1: Develop improved virus expression vectors through characterization of plant viral genomes and investigation of plant virus-host interactions. Objective 2: Evaluate plant and pathogen gene function in host/pathogen interactions and identify and test candidate disease resistance sequences for plant pathogen control. Objective 3: Develop new practical strategies for the production in plants of proteins for prevention, treatment, and control of animal diseases.

Approach:
In Objective 1, we will 1) characterize the genome expression strategy of Maize rayado fino virus and develop into a gene expression tool for maize, and 2) develop novel plant virus-based expression vectors for plants using organelle targeting sequences derived from viroids and viruses. In Objective 2, we will 1) determine the role of membrane-associated, phospholipid-signaling phosphorylation pathways in virus and viroid infection, and 2) evaluate genes with potential for conferring resistance to bacterial pathogens in plants. In Objective 3, we will 1) express novel functional proteins in plants for treatment and control of animal diseases, and 2) develop multi-component vaccines and diagnostic reagents by expression of diverse molecules on the surface of a plant virus-like particle.