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United States Department of Agriculture

Agricultural Research Service

Research Project: Bridging Genomics and Plant Breeding with a Cotton Nested Association Mapping Population Project Number: 5347-21000-011-08
Project Type: Trust

Start Date: Jan 01, 2012
End Date: Dec 31, 2012

Objective:
The primary objectives of this study are to (i) select diverse founders that maximize genetic diversity, and (ii) construct a cotton population of several thousand F5 recombinant inbred lines.

Approach:
A panel of 480 cotton inbred lines was assembled to capture the genes and alleles that form the genetic backbone of modern temperate elite lines. Before selecting diverse founders for constructing a nested association mapping (NAM) population of several thousand F5 recombinant inbred lines, we need to molecularly characterize the genetic structure and diversity among the 480 cotton inbred lines. To that end, we propose to genotype this panel with more than 100 sub-genome specific SSR markers at genome-wide coverage. Not only will this enable definitive selection of founder lines, but also it will provide tremendous insight into the patterns of genetic diversity among different breeding subpopulations. The crossing of diverse founders to a common reference parent will be completed this winter in the greenhouse, followed by selfing of F1 plants in summer 2012. This will allow for the availability of F2 seed for advancement to the F5 generation by single-seed descent in Tecoman from fall 2012 to fall 2013. The large-scale seed increase of all F5 lines would begin in spring 2014. Presently, we are targeting the development of 100-200 RILs for each family. As a genetic complement to the cotton NAM population, a subset of 384 diverse lines will be seed increased in the 2011/2012 Tecoman winter nursery. The other 96 lines of the panel need an additional round of selfing to improve uniformity, followed by seed increasing in Tecoman before inclusion for phenotyping. We will begin phenotyping a single replication (augmented alpha-lattice design) of the 384 lines at NC, SC, and AZ locations in 2012, followed by phenotyping of the 480 lines at the same three locations in 2013. This population will be invaluable as a community resource for genome-wide association studies and training statistical models in genomic selection. Therefore, as with the cotton NAM population, seed of these 480 lines will be deposited in the USDA-ARS National Cotton Collection in College Station, Texas. This will increase the availability of these mapping resources to the global cotton community.

Last Modified: 12/18/2014
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