Project Number: 3635-32000-016-05
Project Type: Specific Cooperative Agreement

Start Date: Apr 17, 2012
End Date: Sep 14, 2013

Objective:
Determine the characteristics of spontaneous avian leukosis-like tumors in chickens.

Approach:
A pyrosequencing assay will be used to assess the TVB genotypes of commercial broiler breeder chickens (200 chickens) in order to determine the genetic susceptibility of the commercial chickens to ALV-E. Using gene expression analysis by microarrays, and by using DNA Southern blotting and hybridization, the tumors induced in chickens (20 samples) will be characterized and the oncogene involved will be determined.