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United States Department of Agriculture

Agricultural Research Service

Research Project: CRYOSTORAGE OF PLANT VARIETY PROTECTION VOUCHER GERMPLASM OF SOLANUM TUBERSOSUM

Location: Plant And Animal Genetic Resources Preservation Research Unit

Project Number: 3012-21000-013-06
Project Type: Reimbursable

Start Date: May 01, 2011
End Date: Sep 30, 2013

Objective:
The objective of the project is to maintain a voucher germplasm of Plant Variety Protection (PVP) potato (Solanum tuberosum) cultivars registered with the U.S. Plant Variety Protection Office. The voucher potato germplasm remains the property of the PVP Office and is securely maintained and kept viable for the duration of the donor’s intellectual property rights. After expiration of the PVP, the potato genetic resources are publically released through the National Plant Germplasm System.

Approach:
The potato cultivars are deposited at the USDA-ARS, National Center for Genetic Resources Preservation as tissue culture and are maintained in vitro as shoots and tubers as well as in long-term liquid nitrogen tanks as shoot tips. The cryopreservation procedure has evolved over years. Currently, the droplet vitrification method developed by Haeng-Hoon et al. (2006) is the widest protocol used. According to the lab standards, a minimum of 40% viability is required for an accession to be considered successfully cryopreserved at the Center. Procedural modifications are made for genotypes not responding to the established protocols.

Last Modified: 12/20/2014
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