Start Date: Sep 14, 2011
End Date: Sep 13, 2014
Ambrosia beetles (Xylosandrus spp. and others) are pests of a wide array of woody plants used in the nursery industry. They depend, for their survival, on their own cultivation of a fungus (Ambrosiella spp. and others). Fungal strains isolated from Xylosandrus spp. will be identified and assessed through sequencing of 18S, 28S and ITS regions of ribosomal DNA from X. crassiusculus and X. germanus. Brood development and symbiont growth will be evaluated for each fungal genotype on representative hosts or diet substrates. Fecundity of beetles within galleries will be evaluated.