Start Date: Aug 01, 2011
End Date: Sep 30, 2012
Successful identification of the hatching factors would enable new approaches to eradicate PCN and potentially even eliminate it as a threat. We will continue our efforts to identify individual hatching factors using LCMS and hatching assays by fractionation of concentrated extracts. Hatching efficacy of fractions will be evaluated at the Kuhl lab at the Univesity of Idaho. We will determine whether crude root exudate can also be used to induce a suicide hatch and production systems to capable of producing large quantities of highly active hatching factors that could be deployed on a commercial scale. We will evaluate different germplasm to see if some genotypes are particularly rich sources of hatching factors. We will examine ways to increase the activity of root exudate. In the Moscow greenhouses, we will test the efficacy of enriched, crude root exudate to cause a suicide hatch in cysts placed in pouches, in pots containing soil similar to the soil types found in Eastern Idaho. We will explore ways to increase the efficacy of potential trap crops, concentrating on S. sisymbriifolium, including ways to increase its root mass to allow better control in infested fields (ie more root mass, deeper root growth) and confirm that any potential trap crop does not allow reproduction of the Idaho G. pallida population.