Location: Obesity and Metabolism Research Unit
Project Number: 5306-51530-019-53
Start Date: Dec 01, 2011
End Date: Mar 31, 2015
Healthy subjects (n=20) will participate in a crossover study with 4 treatment arms: i) control diet with low fiber, ii) low dose (20 g) oligosaccharide added to control diet, iii) high dose (40 g) oligosaccharide added to control diet, and iv) comparison diet with adequate fiber from whole grains. Each treatment will be administered for 3 weeks interspersed with washout periods of 2 weeks. Fecal microbiota will be characterized by sequencing of community 16S rRNA genes. The procedure includes extraction of genomic DNA, amplification of V1 to V3 regions of 16S rRNA genes by PCR, and sequencing using the 454/Roche A sequencing primer kit and Roche 454 GS-FLX Titanium chemistry. Meal challenge protocols will be conducted during the 3rd week of each treatment and fermentation products, glucose control, inflammatory markers and satiety will be assessed. In addition coloncytes will be isolated from fecal samples for the analysis of fermentation products. Measurement of digestible and metabolizable energy will be done by bomb calorimetry and proximate analysis of the controlled treatment diets and coinciding complete urine and fecal collections obtained during the 3rd week of treatment. Biological samples will be archived for future metabolomic analyses using 1H NMR- and mass spec-metabolite profiling, both global & targeted.