Project Number: 5302-22000-010-12
Project Type: Specific Cooperative Agreement

Start Date: Feb 02, 2012
End Date: Aug 31, 2013

Objective:
1. Cultivation of "Ca. L. solanacearum" through in vitro and in planta approaches. 2. Morphological, physiological and genetic characterization of "Ca. L. solanacearum".

Approach:
1. Identify and obtain "Ca. L. solanacearum" infected potato materials from fields in California. Maintain the infected potato in greenhouse. 2. Through grafting, transer the bacterium to different types of plants and evaluate their capacity to host the bacterium. 3. Variate in planta cultivation conditions to optimize bacterial growth. 4. Develop tissue culture, insect cell culture and in vitro culture methods for "Ca. L. solanacearum. 5. Use PCR and electron microscope to monitor the bacterial growth.