Start Date: May 26, 2011
End Date: May 25, 2014
Apply protein engineering to the trichothecene 3-O-acetylase (Tri101) from F. sporotrichioides and F. graminearum. Test the efficacy of the new enzymes in providing resistance to FHB in barley. Correlate the structure and function of the Tri101 protein produced in E. coli with that isolated directly from transgenic barley.