Location: Foreign Disease-Weed Science
Project Number: 1920-22000-041-05
Start Date: Sep 12, 2011
End Date: Sep 11, 2014
Putative resistance genes will be identified through gene mapping, soybean gene expression studies, and from a comparative bioinformatic analysis of the soybean genome. Using virus-induced gene silencing these candidate genes will be silenced, or "turned off", in soybean plants that display resistance towards select isolates of soybean rust. Silenced plants will then be evaluated for a breakdown of resistance by challenging the plants with the pathogen and scoring for the development of disease. A combination of RT-PCR (to assess gene silencing) and gene sequencing will be used to complete the VIGS analysis.