Location: Crop Improvement and Protection Research
Project Number: 5305-22000-013-01
Start Date: Apr 01, 2010
End Date: Sep 30, 2013
Lettuce will be sampled from a diverse array of dieback infested fields from throughout the Salinas Valley and other regions; we will determine if our new molecular detection method can confirm infection more reliably and timely than previous methods. We will test commercial kits for extracting nucleic acid from soils to determine if it is possible to A) detect TBSV and LNSV from soils, and B) quantify levels from different soils and determine correlation with disease incidence in the fields (multi-year objective). Finally, we plan to test a new method for rapid greenhouse pre-screening of lettuce cultivars/lines for tombusvirus resistance.