Start Date: Apr 01, 2011
End Date: Mar 31, 2012
Molecular genetics and plant breeding experiments will be conducted to combine desired mutant alleles controlling the accumulation of different fatty acids in the seed oil. Different crosses will be made between parental lines containing the two genes for the high oleic acid trait and parental lines containing other fatty acid profile traits, including low palmitic acid and elevated stearic acid. Genotyping selections with existing or newly developed molecular marker assays will be performed on the F1 and F2 progeny. Standard population development will be used to advance selected lines to obtain materials for phenotypic evaluation. Field tests will be set up to compare different combinations of alleles important for the seed oil fatty acid profiles to evaluate the effect of each of the alleles working in combination. Laboratory, field, and winter nursery are all components of this research.