Start Date: Apr 01, 2011
End Date: Mar 31, 2013
The entire analytical process will be validated for separate Symphytum sp. based matrices according to the AOAC International Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Sypplements and Botanicals. Standard reference materials will be prepared by isolation and purification of the major pyrrolizidine alkaloids from Symphytum sp. Authentication of standards will include description of characteristics such as physico-chemical properties (e.g. melting point, optical rotation and chromatographic profile), the molecular formula as determined using high resolution mass spectrometry, and a complete structure mapping using nuclear magnetic resonance spectroscopy.