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United States Department of Agriculture

Agricultural Research Service

Research Project: Improvement of Diagnosis of Graft Transmissible Pathogens of Citrus

Location: National Clonal Germplasm Repository for Citrus & Dates

Project Number: 5310-21000-010-02
Project Type: Specific Cooperative Agreement

Start Date: Jun 27, 2011
End Date: Mar 31, 2014

Objective:
Develop cost effective and highly sensitive methods to detect graft transmissible pathogens of citrus.

Approach:
Establish reliable real-time PCR detection of graft transmissible pathogens of citrus including but not limited to Pospiviroids, Hostuviroids, Spiroplasma citri, Dweet mottle virus, citrus tatter leaf virus. Establish protocols for rapid extraction of pathogen nucleic acid from target tissue. Develop genomic information of graft transmissible pathogens of citrus including pathogens which are presently uncharacterized.

Last Modified: 7/28/2014
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