Start Date: Jun 27, 2011
End Date: Mar 31, 2014
Establish reliable real-time PCR detection of graft transmissible pathogens of citrus including but not limited to Pospiviroids, Hostuviroids, Spiroplasma citri, Dweet mottle virus, citrus tatter leaf virus. Establish protocols for rapid extraction of pathogen nucleic acid from target tissue. Develop genomic information of graft transmissible pathogens of citrus including pathogens which are presently uncharacterized.