Location: Tick and Biting Fly Research
Project Number: 6205-32000-031-34
Start Date: Mar 01, 2011
End Date: Dec 31, 2013
A controlled stall trial for vaccination of cattle and tick challenge will be conducted to determine if the receptor fragments can protect cattle against the tick. Determine if the receptor antigen produces an immunogical response in cattle as it did in rabbits. Cattle will be inoculated with a mixture of synthetic peptides encompassing receptor extracellular regions and part of the transmembrane regions; peptides will be linked to keyhole limpet hemocyanin. If cattle develop anti-receptor (peptide) antibodies, cattle will be challenged with ticks; tick survival, weight, egg laying capacity, and egg viability will be measured and statistically compared with controls. Antibodies against the cloned receptors will be developed also in rabbits for the immunolocalization in different tick tissues, ideally also following a temporal-spatial analysis, depending on the availability of tick females at different ages. For objective 2.B: active myokinins will be injected directly into the female tick.