Novel Real-Time PCR-Based Surveillance Systems for Candidatus Liberibacter Species, Bacteria Associated with Citrus Huanglongbing
Project Number: 5302-22000-010-06
Start Date: Dec 01, 2010
End Date: Nov 30, 2012
1. To develop ultra sensitive Taq-Man PCR diagnostic system for improved detection of Ca. Liberibacter asiaticus.
2. To design a Ca. Liberibacter genus-specific real-time PCR technology for general detection of Candidatus Liberibacter species.
1. Cloning, sequencing and characterizing genomic sequences of citrus Candidatus Liberibacter species using novel molecular technique.
2. Design and validate sensitivity and specificity of diagnostic systems.