Project Number: 5302-22000-010-07
Project Type: Reimbursable

Start Date: Sep 01, 2010
End Date: Aug 31, 2014

Objective:
Design a ZC specific realtime PCR diagnosis for reliable detections of ZC-infected potato and potato psyllid. 2. Design and develop multi-locus DNA Simple Sequence Repeat (SSR) DNA markers to genotype and assess genetic diversity of ZC-associated Liberibacter bacteria.

Approach:
1. Clone and sequence ZC Liberibacter pathogen to identify suitable sequencing regions for designing realtime PCR. Validate sensitivity and specificity of detection. 2. Conduct whole genome analyze of ZC Liberibacter draft genome and identify SSR loci for designing SSR primers. 3. Conduct population genetic analysis of ZC-associated Candidatus Liberibacter and assess genetic diversity of the pathogen.