Location: Beneficial Insects Introduction Research
Project Number: 1926-22000-023-10
Start Date: Oct 01, 2010
End Date: Sep 29, 2014
We will use the tropical ash-based method to rear EAB larvae for tests with two different species of EAB larval parasitoids: T. planipennisi (a gregarious endoparasitoids) and Spathius sp (a newly imported gregarious, ectoparasitoid). Freshly cut tropical ash logs will be infested with fertilized EAB eggs laid on strips of un-bleached coffee filter paper. EAB egg-infested logs will be then placed into ventilated plastic boxes, and incubated under normal rearing conditions (27oC; RH 45 - 65%, and photoperiod L:D=16:8 hrs) prior to use in different tests for Objectives 1 and 2. A standardized size of the tropical ash logs (2.5 cm in diameter x 20 cm in length) will be used to rear EAB larvae for different tests.