Evaluation of Myb1a Gene Expression in Citrus
Subtropical Insects and Horticulture Research
Project Number: 6618-21000-014-03
Nonfunded Cooperative Agreement
Start Date: Dec 01, 2010
End Date: Sep 30, 2015
Evaluate potential of myb1A gene constructs (from Vitis vinifera) for use in producing anthocyanin in citrus. If effective, the citrus gene equivalent will be extracted and utilized as a cis-genic selectable marker.
Use two constructs obtained from the collaborator in Agrobacterium mediated transformation of citrus and tobacco. Regenerate shoots and assess for presence of myb1A and evaluate gene expression. If promising plants will be grown out for further assessment.