Project Number: 6625-21000-003-13
Project Type: Nonfunded Cooperative Agreement

Start Date: Jan 01, 2011
End Date: Dec 31, 2012

Objective:
Develop and test technology and methodology towards genomic based Single Nucleotide Polymorphism (SNP) marker discovery and trait association in sugar cane.

Approach:
The parties will test whether a sequence based bulk segregant analysis (seqBSA) pooling strategies, methods, and analytical algorithms developed and validated in other crops can be applied to sugar cane for both marker discovery and trait association using the Solexa GA2 technology platform. Solexa sequence data will be generated by the cooperator from DNA/RNA bulked from genotypes selected by ARS and segregating for resistance to brown and orange rust of sugarcane as well as their respective parents. Criteria used by ARS to select the populations will be as follows: High quality and accurate and Replicated spatially and/or over time. The Cooperator will evaluate Solexa sequencing for marker discovery, mapping and marker trait association using sugar cane material and phenotypic data.