Location: Agroecosystem Management Research
Project Number: 5440-12630-001-01
Start Date: Oct 01, 2010
End Date: Jul 31, 2013
Sub-objective #1, Normal VTS operation: …during 2010 and 2011… [add to the end]--Pooled samples of ‘clean’ rainwater runoff (two per event), feedlot runoff (four per event) and excess infiltration runoff (two per event) will be analyzed in the University of Nebraska Water Science Laboratory for hormones (nearly three dozen, including estradiols, testosterones, progesterones, estriols, and trenbolone) and antibiotics (tetracyclines/sulfonamides and macrolide/betalactams). These samples will also be screened for resistance the third generation antibiotic ceftiofur that is widely used in cattle (Excede, Naxcel) for problems such as foot rot or eye infections. Sub-objective #2, Movement and persistence of nutrients and pathogens and fecal indicator organisms: Once each year, wastewater will be applied to four of the VTA treatment cells followed by collection of surface soil samples from five sites within each of the four cells and then sample those sites periodically over two weeks in order to understand how constituents in the wastewater are utilized, transformed, or removed. Soil depth profiles down to 30 cm (5 cm increments) will also be determined at those five sites in each of the four cells to help gauge the downward movement of microbes and nutrients. The 30-cm soil profile will be collected once each year to determine the movement of constituent over the two-year study.