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United States Department of Agriculture

Agricultural Research Service

Research Project: GENOME-WIDE DISCOVERY OF THE GENES/LOCUS DETERMINING THE OIL COMPOSITION AND OIL "FUNCTIONAL" MARKERS BY EXPLORING SOYBEAN GENETIC DIVERSITY

Location: Plant Genetics Research

Project Number: 5070-21000-037-01
Project Type: Reimbursable

Start Date: Apr 01, 2011
End Date: Mar 31, 2014

Objective:
1. Determine DNA sequences and expression levels of genes expressed in soybean seeds from fourteen genotypes. 2. Discover variations of gene sequences and expression levels in soybean seeds of the fourteen genotypes. 3. Discover a set of soybean oil "functional" markers for the fourteen genotypes.

Approach:
Overall objective of the collaborative research proposal is to identify oil and meal traits and genes that influence those traits to improve the quality and value of US Soybean in the target area of composition. The proposed research will use genomics and bioinformatics approaches to predict the gene important for oil storage composition and identify gene markers to facilitate oil gene discovery. The proposed research will select fourteen genotypes containing diverse levels of oleic acid, linolenic acid and stearic acid levels, and determine their seed storage lipid profiles. In addition, deep sequencing technology will be used to determine sequences and accumulation levels of genes expressed in seeds from the fourteen genotypes and to identify their sequence and/or accumulation level variations among the genotypes. Association and a variety of data mining approaches will be used to predict the genes potentially regulating and participating in seed storage oil production. The sequence and expression variations that occur in those oil related genes will be further developed as a set of oil "functional" markers.

Last Modified: 10/24/2014
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