MOLECULAR CHARACTERIZATION OF FIELD POPULATIONS OF GLOBODERA PALLIDA FROM IDAHO AND DEVELOPMENT OF NOVEL NEMATODE RESISTANCE IN POTATO
Biological Integrated Pest Management Unit
Project Number: 1907-22000-021-12
Specific Cooperative Agreement
Start Date: Sep 24, 2010
End Date: Mar 23, 2012
The goal of this cooperative research project is to understand the genetic complexity of field populations of the emerging species of potato cyst nematode (PCN) and to develop novel control tools to aid the eradication of PCN in Idaho. Specific objectives include: 1) use the chorismate mutase gene as a genetic marker to evaluate genetic variations among PCN field populations, 2) develop novel nematode resistance in potato through a plant-delivered RNAi technology.
The chorismate mutase gene will be cloned from different PCN field populations and the obtained gene sequences will be analyzed and compared to discover genetic variations. Transgenic potato lines expressing dsRNA targeting different parasitism genes will be generated and tested for nematode infection.