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United States Department of Agriculture

Agricultural Research Service

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Research Project: Improved Utilization of Ag. Products Through Identification of Nitrogen-Containing Bioactive Components Important to Quality & Human Health

Location: Healthy Processed Foods Research

Project Number: 5325-41430-011-00
Project Type: Appropriated

Start Date: Jun 13, 2010
End Date: Jun 12, 2015

Objective:
Nitrogen-containing plant metabolites are an important class of natural products that contribute to quality and utilization and span the range from essential nutrients to phytochemicals that affect mood and mental well being and, in some extreme cases, toxins. Due to complexity and difficulty in detection, past research in this area has targeted very specific compounds, resulting in most nitrogen-containing plant metabolites being largely ignored. Objective 1- Develop new or improved existing methods to detect, identify, and characterize N-containing plant metabolites. Establish extraction methods for N-containing metabolites from fruit of specialty crops (e.g., citrus, grapes, and tomatoes) and model plant species. Establish High Pressure Liquid Chromatography (HPLC) separation method for the resolution of multiple classes of nitrogen-containing metabolites in a single run. Compare existing methods for the detection and quantification of nitrogen-containing metabolites. Combine the elements of extraction, separation, identification and quantification into an optimized method for the profiling of N-containing plant metabolites. Objective 2- Screen specialty crops for their metabolomic profiles with a particular emphasis on nitrogen-containing metabolites. Initial efforts will focus on fruits from citrus, grapes, and tomatoes. Sub-Objective 2.1. Commence NMP and systematic identification and quantification of nitrogen-containing metabolites found in tissues of citrus, grapes, and tomatoes, using liquid chromatography coupled to mass spectrometer (MS & MS/MS) and nitrogen (CND and/or post column derivatization) detection systems. Sub-objective 2.2. Isolate and/or identify new or novel nitrogen-containing metabolites.

Approach:
1) Develop new or improve existing methods to detect, identify, and characterize N-containing plant metabolites. 2) Screen specialty crops for their metabolomic profiles with a particular emphasis on nitrogen-containing metabolites. Initial efforts will focus on the fruits from citrus, grapes, and tomatoes.

Last Modified: 9/20/2014
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