Start Date: Aug 18, 2010
End Date: Dec 31, 2013
Microarray technology currently allows the analysis of all exons in a gene so that all alternative splicing can be detected. In a pioneering experiment we have identified differences in genes and alternatively spliced genes between normal and insulin resistant obese mice by exon microarray. The expression of these genes must be confirmed by PCR and a pathway analysis of the relationship between these genes and insulin resistance needs to be developed assisted by metabolic pathway analysis software such as GeneSpring.