Location: Cereal Disease Laboratory
Project Number: 5062-21220-021-06
Start Date: Feb 10, 2010
End Date: Jan 31, 2013
This project will focus on developing or adapting diagnostic protocols for the following high-impact plant pathogens: 1) Raffaellea lauricola; 2) Puccinia graminis f.sp. tritici; 3) Phytophthora ramorum; 4) Candidatus Liberibacter asiaticus; 5) three major virus genera (Crinivirus, Begomovirus and Potyvirus). Available diagnostic protocols based on PCR will be tested and modified where necessary in order to adapt to a plant diagnostic clinic setting. Positive controls will be developed for each of the assays. DNA extractions methods will be improved and modified for efficient and high-throughput laboratories. Internal controls will be developed to verify assay conditions. Bead-based PCR reagents will be developed. Assays will be verified in test laboratories. Training activities (workshops, development of online materials and printed materials) will be developed and deployed for diagnosticians.