Page Banner

United States Department of Agriculture

Agricultural Research Service

Related Topics

Research Project: IMPROVEMENT AND DEPLOYMENT OF RAPID STANDARDIZED PCR DIAGNOSTIC TOOLS TO INCREASE DETECTION CAPACITY FOR HIGH-IMPACT PLANT PATHOGEMS

Location: Cereal Disease Laboratory

Project Number: 3640-21220-021-06
Project Type: Reimbursable

Start Date: Feb 10, 2010
End Date: Jan 31, 2013

Objective:
The long-term goal of this integrated project is to increase early and accurate detection of high-impact plant pathogens. The specific objectives are: 1) Research – Adaptation of existing plant pathogen protocols for use in diagnostic laboratories and the production of high-quality, standardized controls; 2) Extension – Develop and disseminate disease-specific sample submission information to increase the quality and quantity of important plant disease samples; 3) Evaluation – Determine the success and impact of the research and extension portions by using surveys of end-users and assessment of data generated by research and extension projects.

Approach:
This project will focus on developing or adapting diagnostic protocols for the following high-impact plant pathogens: 1) Raffaellea lauricola; 2) Puccinia graminis f.sp. tritici; 3) Phytophthora ramorum; 4) Candidatus Liberibacter asiaticus; 5) three major virus genera (Crinivirus, Begomovirus and Potyvirus). Available diagnostic protocols based on PCR will be tested and modified where necessary in order to adapt to a plant diagnostic clinic setting. Positive controls will be developed for each of the assays. DNA extractions methods will be improved and modified for efficient and high-throughput laboratories. Internal controls will be developed to verify assay conditions. Bead-based PCR reagents will be developed. Assays will be verified in test laboratories. Training activities (workshops, development of online materials and printed materials) will be developed and deployed for diagnosticians.

Last Modified: 8/30/2014
Footer Content Back to Top of Page