Location: Plant Science Research
Project Number: 6645-11000-008-00
Start Date: Jun 02, 2010
End Date: Jun 01, 2015
Experiments will be conducted in available open top field chambers, greenhouse exposure chambers, and custom Outdoor Plant Environment Chambers (OPECs) or in a new air exclusion system to be developed by this project that allow for testing of plant responses to combinations of carbon dioxide and ozone under contrasting conditions of temperature and vapor pressure deficit. A multi-year field study will be established using the air exclusion system to test the effects of elevated ozone, temperature and carbon dioxide on a soybean-winter wheat continuous no-till cropping system. Detailed assessments of plant growth, biomass, and yield along with measurements of leaf gas exchange, tissue chemistry and micrometeorological data will be used as inputs for parameterization of DSSAT-CSM CROPGRO-Soybean and CERES-Wheat models. Ozone-sensitive and tolerant snap beans will be grown in the OPECs where control of relative humidity and temperature allows for the study of plant responses to elevated ozone and carbon dioxide under contrasting vapor pressure deficit conditions. Wheat cultivars that are susceptible and resistant to stripe or stem rust will be grown in the OPECs and inoculated with pathogens under a range of carbon dioxide, ozone, temperature and vapor pressure deficit conditions to examine the potential impact of these climate change factors on infection and progression of disease. Soybean germplasm will be exposed to elevated ozone conditions in greenhouse exposure chambers or open-top field chambers and foliar injury and seed yield measurements used to identify tolerant cultivars. Single nucleotide polymorphism markers will be applied to a soybean population developed from a cross between ozone-sensitive and tolerant soybean ancestors and the population screened for ozone-induced foliar injury in the greenhouse. Marker and phenotype data will be combined to develop a map of soybean ozone-tolerance genes.