Project Number: 5305-22000-012-18
Project Type: Specific Cooperative Agreement

Start Date: Aug 13, 2010
End Date: Jun 30, 2012

Objective:
The specific objectives of this project are to 1) develop a rapid detection assay for Verticillium dahliae in spinach seed; 2) determine where the fungus is located in spinach seed to develop effective seed treatments; and 3) identify lettuce and spinach resistant to V. dahliae to limit spread via seed.

Approach:
Detection of the fungus in vegetable and spinach seeds will be carried out by PCR techniques; localization of the fungus in spinach seed will be determined by confocal microscopy; and spinach and lettuce will be screened for resistance to Verticillium wilt in greenhouses. Documents SCA with University of California Davis