OAT SNP DEVELOPMENT & IDENTIFICATION OF LOCI AFFECTING KEY TRAITS IN NORTH AMERICAN OAT GERMPLASM USING ASSOC...CANADIAN INVOLVEMENT
Location: Small Grains and Potato Germplasm Research
Project Number: 5366-21000-028-25
General Cooperative Agreement
Start Date: Apr 01, 2010
End Date: Aug 31, 2012
Objective 1: Develop a highly informative DArT-MAB genotyping platform complementary to the OOPA-MAB platform and benchmark both platforms. Objective 2: Conduct a two year multi-location association mapping study to develop marker-trait associations to facilitate the development of new oat varieties with superior health benefits and improved agronomics, milling quality, and disease resistance. Objective 3: Partner with the barley CAP portal to effectively utilize and disseminate the information generated by the project.
1. Genotyping: Two DArT discovery arrays from the PstI/TaqI genomic representation will be developed. The arrays will be used to genotype an oat reference panel, and at least one mapping population. From these, we will choose a robust and informative subset of 3,000 final DArT markers that will be arrayed on a second-generation DArT genotyping platform (DArT-MAB). 2. Field testing: A single row augmented plot design will be used to evaluate the oat association mapping population and checks in Winnipeg and Ottawa. Agronomic traits evaluated prior to harvest will include; 1) heading date, 2) plant height, 3) lodging, and 4) maturity. Each location will collect 500g of seed for shipment to Aberdeen, ID, where all processing and preparation for quality and nutritional component evaluation will occur. Quality traits measured in Aberdeen will include: 1) groat percentage, 2) percentage plump kernels, 3) 1000 kernel weight, 4) groat plumpness, 5) groat color, and 6) percentage broken groats. 3. Qaulity: 50 grams of ground meal from groats from two locations over two years will be sent to Winnipeg for determination of total dietary fiber and ß-glucan using standardized methods. 4. Data management: POOL will be expanded to contain the germplasm from this project, and will coordinate records so that they are mirrored in THT.